## Codes for vectors and associated cloning sites

Most clones are defined by their initial letter(s), according to the
table below.

The principal exceptions are:

1) Laboratory designations (2 letters followed by a number)

2) MIT clones, which have 1, 2, or 3 identical letters, followed by any
letter from A to H, followed by a number from 1 to 12. Vector is pHC79.
Method of selection:

pJB8 and pHC79 ampicillin

Lorist kanamycin

pYAC4 yeast ura-medium

G pfosl chloramphenicol

Note that in most cases Lorist clones are the easier cosmids to grow;
because, unlike ampicillin, kanamycin is not destroyed by the cells.

Clone name: Vector:
A Lambda 2001 Sau3AI partial
ZL Lambda 2001 Sau3AI partial probed
YSL Lambda 2001 YAC subclones, Sau3AI partial
B pJB8 serial Sau3AI partial
C pJB8 gridded in microtiter plates Sau3AI partial
D pJB8 gridded on filters Sau3AI partial
E pJB8 EcoK minus Sau3AI partial
R pJB8 RI partial
M pJB8 MboI partial
ZC pJB8 probed
K Lorist B Sau3AI partial
T Lorist 2 Sau3AI partial
W Lorist 6 NotI plus Sau3AI partial
F Lorist 6 Sau3AI partial
ZK 15 - 56 Lorist 6 Sau3AI partial probed
57 - 130 Lorist 2 Sau3AI partial probed
131 - 164 Lorist 6 Sau3AI partial probed
165 - 177 Lorist 2 Sau3AI partial probed
178 - 192 Lorist 6 Sau3AI partial probed
193 - 344 Lorist 2 Sau3AI partial probed
345 - 354 Lorist 6 Sau3AI partial probed
355 - 514 Lorist 2 Sau3AI partial probed
515 - 552 Lorist 6 Sau3AI partial probed
553 - 596 Lorist 2 Sau3AI partial probed
597 - 616 Lorist ? Sau3AI partial probed
617 - 626 Lorist 2 Sau3AI partial probed
627 - 692 Lorist 6 Sau3AI partial probed
693 - 756 Lorist 2 Sau3AI partial probed
757 onwards Lorist 6 Sau3AI partial probed
Y pYAC4 RI partial
Yeast:
PEL pel61 SmaI
9... pphc79 SmaI
Additional information
---------- -----------
Name: Vector: Cell: Selection: Cloning site: Organism:
H_DJ pcypac2 DH10B kan Sau3A in BamH1 (GATC) Human(RPCI1,3-5)
G pfos1 XL1 chlor Sau3A in BamH1 (GATC) C. briggsae
H pfos1 XL1 chlor Sau3A in BamH1 (GATC) C. elegans
MM lorist6 XL1 kan Sau3A in BamH1 (GATC) C. briggsae
H_GS pbelo DH10B chlor HindIII (AAGCTT) Human
H_RG pbelo DH10B chlor HindIII (AAGCTT) Human
H_LUCA pwe15 ? kan EcoRI (GAATTC) Human (III)
H_P1 p1 ? kan Sau3A in BamH1 (GATC) Human (III)
H_U lawrist16 ? kan Sau3A in BamH1 (GATC) Human (X)
H_LL lawrist16 ? kan Sau3A in BamH1 (GATC) Human (7)
A_TM pbelo ? chlor HindIII (AAGCTT) Arabidopsis
A_IG pbelo ? kan EcoRI (GAATTC) Arabidopsis
H_NH pBACe3.6 DH10B chlor EcoRI (GAATTC) human (RPCI11)
H_DA pPAC4 DH10B kan MboI in BamHI (GATC) human (RPCI6)
CB pBAC3.6 DH10B chlor EcoRI (GAATTC) C. briggsae