ÿþ<HTML> <HEAD> <!-------------- Head --------------> <TITLE> Electron Microscopy in Structural Biology </TITLE> <META NAME="CREATOR" CONTENT="Johan Hebert"> <META NAME="KEYWORDS" CONTENT="scandem"> <META NAME="DESCRIPTION" CONTENT=""> <!-------------- End Head --------------> </HEAD> <!-------------- Body and Style Configuration --------------> <BODY BGCOLOR="#FFFFFF" TEXT="#000000" LINK="#3399FF" VLINK="#3399FF" ALINK="#3399FF"> <STYLE> A:hover {color: #FF0000; text-decoration:none;} A:visited {font-family: Verdana,Arial,Helvetica,sans-serif; font-size: 10px; text-decoration:none;} A:link {font-family: Verdana,Arial,Helvetica,sans-serif; font-size: 10px; color: #3399FF; text-decoration:none;} </STYLE> <FONT FACE="Geneva, Verdana, Arial, Sans-serif" SIZE="2"> <!-------------- End Body and Style Configuration --------------> <!-------------- Table --------------> <TABLE CELLPADDING="0" CELLSPACING="0" BORDER="0"> <TD WIDTH="720px" VALIGN="TOP"> <FONT FACE="Verdana, Arial, Helvetica" SIZE="2"> <IMG SRC="bilder/ki.jpg"> <IMG SRC="bilder/emg.gif" WIDTH="500" HIGHT="75"> <IMG SRC="bilder/csb.gif"> <BR> </TD><TR> <TD WIDTH="720px" VALIGN="TOP" BACKGROUND="bilder/line.gif"> <FONT FACE="Verdana, Arial, Helvetica" SIZE="2"> </TD><TR> <TABLE CELLPADDING="0" CELLSPACING="0" BORDER="0"> <TD WIDTH="170px" VALIGN="TOP"> <FONT FACE="Verdana, Arial, Helvetica" SIZE="1"> <BR> <A HREF="index.html">Home</A><BR> <B>General</B><BR> &nbsp;<A HREF="resprof.html">Research profile</A><BR> &nbsp;<A HREF="equipment.html">Equipment</A><BR> &nbsp;<A HREF="personnel.html">Personnel</A><BR> &nbsp;<A HREF="publications.html">Publications</A><BR> <BR><B>Projects</B><BR> &nbsp;<A HREF="l_assemblies.html">Large assemblies</A><BR> &nbsp;<A HREF="m_permease.html">Melibiose permease</A><BR> &nbsp;<A HREF="m_proteins.html">Membrane proteins</A><BR> &nbsp;<A HREF="mgst.html">MGST</A><BR> &nbsp;<A HREF="na_k_atpase.html">Na,K-ATPase</A><BR> &nbsp;<A HREF="p_e2_synthase.html">Prostaglandin E2 synthase</A><BR> <BR><B>Events</B><BR> <BR><B>Miscellaneous</B><BR> &nbsp;<A HREF="links.html">Links</A><BR> </FONT> </TD> <TD WIDTH="550px" VALIGN="TOP"> <FONT FACE="Verdana, Arial, Helvetica" SIZE="2"> <BR> <B>Publications from the EM group at CSB, 1997 - 2003</B><BR><BR> Ellis, M. and Hebert, H. & Thelestam, M. (1997) <I>Staphylococcus aureus</I> alpha-toxin: Characterization of Protein/Lipid Interactions, 2D crystallization on Lipid Monolayers and 3D Structure, <I>J. Struct. Biol.</I>, <B>118</B>, 178-188.<BR><BR> Xian, Y. & Hebert, H. (1997) Three-Dimensional Structure of Porcine Gastric H,K-ATPase From Negatively Stained Crystals, <I>J. Struct. Biol.</I>, <B>118</B>, 169-177.<BR><BR> Hebert, H., Schmidt-Krey, I., Morgenstern, R., Murata, M., Hirai, T., Mitsuoka, K. & Fujiyoshi, Y. (1997) The 3.0 Å Projection Structure of Microsomal Glutathione Transferase as Determined by Electron Crystallography of p21212 Two-dimensional Crystals, <I>J. Mol. Biol.</I>, <B>271</B>, 751-758.<BR><BR> Ellis, M.J., Knapp, S., Koeck P.J.B., Fakoor-Biniaz, Z., Ladenstein, R. & Hebert, H. (1998) Two-dimensional Crystallization of the Molecular Chaperonin TF55 from the Hyperthermophilic Archaeon <I>Sulfolobus solfataricus. J. Struct. Biol.</I>, <B>123</B>, 30-36.<BR><BR> Schmidt-Krey, I., Lundqvist, G., Morgenstern, R. & Hebert, H. (1998) Parameters for the two-dimensional crystallization of the membrane protein microsomal glutathione transferase. <I>J. Struct. Biol.</I>, <B>123</B>, 87-96.<BR><BR> Koeck, P.J.B., Hebert, H., Ellis, M.J., Ladenstein, R. & Trent, J.D. (1998) Two-dimensional Crystals of Reconstituted Beta-subunits of the Chaperonin TF55 from <I>Sulfolobus Shibatae. Biochim. Biophys. Acta</I>, <B>1429</B>, 40-44.<BR><BR> Hebert, H. (1998) Two-dimensional Crystals of Membrane Proteins. In Biomembrane Structures (Eds. D. Chapman and P. Haris), IOS Press, pp. 88-110.<BR><BR> Schmidt-Krey, I., Murata, K., Hirai, T., Morgenstern, R., Fujiyoshi, Y. & Hebert, H. (1999) The Projection Structure of the Membrane Protein Microsomal Glutathione Transferase at 3 Å Resolution as Determined from Hexagonal Two-dimensional Crystals, <I>J. Mol. Biol.</I>, <B>288</B>, 243-253.<BR><BR> Hesse, J., Hebert, H. and Koeck, P.J.B. (2000) Evaluation of Scanners and Comparison to CCD Cameras for High-Resolution TEM of Protein Crystals and Single Particles, <I>Microsc. Res. Tech.</I>, <B>49</B>, 292-300.<BR><BR> Schmidt-Krey, I., Mitsuoka, K., Hirai, T., Murata, K., Cheng, Y., Fujiyoshi, Y., Morgenstern, R. and Hebert, H.(2000) The three-dimensional map of microsomal glutathione transferase 1 at 6 Å resolution. <I>EMBO J</I>. <B>19</B>, 6311-6316.<BR><BR> Hebert, H., Xian, Y., Thomsen, K. And Maunsbach, A.B. (2000) Structure of Renal Na,K-ATPase as observed by cryo-EM of 2-D crystals. In: Na/K-ATPase and Related ATPases, eds. Taniguchi, K. and Kaya, S., Elsevier Science, Amsterdam, pp 43-48.<BR><BR> P.J.B. Koeck (2000):  Ins and Outs of Digital Electron Microscopy ; <I>Microscopy Research and Technique</I> <B>49</B>, 217-223.<BR><BR> P. J. B. Koeck (2000):  Quantization Errors in Averaged Digitized Data ; <I>Signal Processing</I> <B>81</B>, 345-356.<BR><BR> Hebert, H., Purhonen, P., Vorum, H., Thomsen, K. and Maunsbach, A.B. (2001) Three-dimensional structure of renal Na,K-ATPase from cryo-electron microscopy of two-dimensional crystals. <I>J. Mol. Biol.</I> <B>314</B>, 479-494.<BR><BR> Ellis, M.J. and Hebert, H. (2001) Structure analysis of soluble proteins using electron crystallography, <I>Micron</I>, <B>32</B>, 541-550.<BR><BR> Ek. M., Engblom, D., Saha, S., Blomqvist, A., Jakobsson, P.J., Ericsson-Dahlstrand, A. (2001) Inflammatory response:Pathway across the blood-brain barrier. <I>Nature</I>, <B>410</B>, 430-431.<BR><BR> Hacksell, I. Rigaud, J.-L., Purhonen, P., Pourcher, T., Hebert, H. and Leblanc, G. (2002) Projection structure at 8 Å resolution of the melibiose permease, an Na-sugar co-transporter from <I>Escherichia coli, EMBO J.</I>, <B>21</B>, 3569-3574.<BR><BR> Holm, P., Morgenstern, R. and Hebert, H. (2002) The 3-D structure of microsomal glutathione transferase 1 at 6 Å resolution as determined by electron crystallography of p22121 crystals. <I>Biochim. Biophys. Acta</I>, <B>1594</B>, 276-285.<BR><BR> Engblom, D., Ek. M., Saha, S., Ericsson-Dahlstrand, A., Jakobsson, P.J., Blomqvist, A. (2002) Prostaglandins as inflammatory messengers across the blood-brain barrier. <I>J. Mol. Med.</I> <B>80</B>, 5-15.<BR><BR> Hebert, H., Purhonen, P., Thomsen, K., Vorum, H. and Maunsbach, A.B. (2003) Renal Na,K-ATPase Structure from Cryo-electron Microscopy of Two-Dimensional Crystals. <I>Ann N.Y. Acad. Sci.</I> <B>986</B>, 1-9.<BR><BR> Thorén, S., Weinander, R., Saha, S., Jegerschöld C., Pettersson P.L., Samuelsson, B., Hebert, H., Hamberg, M., Morgenstern, R., Jakobsson, P. J. (2003) Human Microsomal Prostaglandin E Synthase-1: Purification, Functional Characterization and Projection Structure Determination. J. Biol. Chem. In press.<BR><BR> </FONT> </TD><TR> <TD> </TD> <TD VALIGN="BOTTOM"><BR> <FONT FACE="Verdana, Arial, Helvetica" SIZE="1"> <BR> <CENTER> Last Updated: Friday, January 30, 2004 <BR> &#169;2003 <A HREF="mailto:johanhebert@hotmail.com">Webmaster</A>. All rights reserved. </CENTER> </FONT> </TD> </TABLE> </TABLE> <!-------------- End Table --------------> </BODY> </HTML>