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Equipment Structural studies by the use of cryoEM can be divided into several steps from production of suitable specimens to analysis using image processing tools. The following equipment is available: Labs well equipped for molecular biology and protein chemistry work Equipment for producing EM specimens, for instance a carbon evaporator and a plunge freezing device. An FEI 120 kV electron microscope with several cryo holders A JEOL 3000SFF electron microscope with a liquid helium cooled specimen stage (This microscope is located at our affiliated lab in Lund, 4 hours from the CSB lab). Tietz CCD cameras attached to the microscopes. Dark room facilities for film handling. An optical bench for quick evaluation of films. A Zeiss SCAI scanner. Computers and software for processing both crystal data (MRC package) and images of single particles (Spider, EMAN).

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